The type of chemicals separated on a GreenSep Pyridyl Amide column would normally require the addition of TFA or an amine to the mobile phase as a peak shape modifier; however GreenSep Pyridyl Amide does not require this addition. Ideal for chemicals that contain both basic amine and acidic groups, as shown in the chromatogram below, GreenSep Pyridyl Amide provides flexibility for the SFC chromatographer with mobile phase composition and fraction collection greatly simplified without the use of amino additives.