Supercritical fluid chromatography (SFC) is a powerful chromatographic technique for the separation of complex mixtures. It has been useful in the areas of preparative chromatography and rapid analysis chromatography. Many SFC separations have been forced to utilize older types of stationary phases from “normal phase” HPLC such as unmodified silica, diol, amino and cyano. These phases are poorly adapted to SFC and present a number of limitations for SFC separations. Limitations include: low capacity, poor selectivity, and poor peak shape for SFC separations.
