The chromatographic separation of chiral compounds is an important tool in the search for new pharmaceutical entities. Both HPLC and SFC separations of chiral chemicals are important tools for analytical determination and preparative isolation of enantiomeric mixtures. Existing chiral stationary phases can separate a wide variety of chiral mixtures. However there are still enantiomeric mixtures that are difficult to separate limiting the characterization of these mixtures. Difficult to separate enantiomeric mixtures require chiral stationary phases with increased separation power such as our newly developed phase: ChromegaChiral CCU is our newest product for high resolution chiral separations. It is a modified amylose coated on the high purity, high performance spherical silica particles. The chemical modification includes the combination of chemical bonding groups of methylbenzylcarbamate and 3-chloro-4 methylphenylcarbamate attached to amylose. This combination of bonded groups stabilizes the solubility of coated phase making for a durable phase similar to other widely used coated phases. The use of amylose modified with phenyl groups moiety provides for the separation for many previously unresolved/poorly resolved chiral mixtures. This chemical modification provides for unique separation behavior. A baseline chiral separation of the herbicide Benoxacor on this unique phase is shown below.
