Traditional HILIC Phase
Reversed-phase HPLC is widely used for separation of many pharmaceutical compounds. However, retention and separation of many polar analytes has proven to be a challenge. Many of these types of compounds are unretained or poorly retained on most conventional reversed-phase stationary phases, such as ODS. Fortunately, to deal with these types of analytes an alternative mode of chromatography can be utilized using polar stationary phases and highly organic mobile phases. This mode of chromatography has been utilized for many years and is referred to as “hydrophilic interaction chromatography” or HILIC. HILIC chromatography uses mobile phases containing between 5 - 20 % water for the retention of polar compounds. ES Industries is pleased to offer a number of stationary phases based traditional bonded phases such as amine and diol. The columns are produced from ultra high purity silica using the most advanced bonding technology available. In addition, the columns are packed to the highest possible standards yielding superior performance and durability. The following traditional HILIC phases are available from ES Industries.