ES Industries has developed columns specifically optimized for LC-MS analysis which exhibit high efficiencies and excellent reproducibility, two characteristics essential for high performance LC-MS. While any of our column products may be purchased for LC-MS applications we have developed several column kits specifically for LC-MS. The packings in these kits are carefully selected to meet the high performance demands of LC-MS. Detailed descriptions of each column chemistry contained in the kits are found on pages 6 and 27. The use of each column chemistry for a particular sample, mobile phase composition or matrix can be briefly categorized as follows: 54

WR-C8 or WR-C18 are a base deactivated column that can analyze both acidic and basic compounds that contain some nonpolar functionality. This column should be used with mobile phases containing at least 10% organic solvent.

ProTec C18 is a base deactivated column that enables the analysis of basic compounds without the use of amine modified mobile phases. This column is excellent for compounds containing amine groups. ProTec-RP can often produce better peak shapes for amine compounds than Chromegabond WR and can be used with 100% aqueous mobile phases.

AquaSep has been developed using patented technology for use with highly aqueous mobile phases, including 100% aqueous. Our patented bonding chemistry allows the bonded C8 chains to remain fully extended in the mobile phase even under highly aqueous conditions. AquaSep can retain highly water soluble compounds such as small organic acids, water soluble vitamins, and low molecular weight polar compounds. It can eliminate the need for ion-pairing reagents.

FluoroSep-RP Phenyl (FSP) contains monomerically bonded pentafluorophenyl groups. The pentafluorophenyl groups interact with analytes via pi-pi electron retention process. This phase is useful for the separation of halogen containing compounds, aromatics, conjugated systems, and epimers. These electron interactions are found in many natural product mixtures.

ES Industries LC-MS Kits contain column dimensions (5 cm x 2 mm) that are designed to deliver maximum sensitivity and rapid analysis.