The Epic Polar XP ODS phase has been specifically developed using patented technology for use with highly aqueous mobile phases, including 100% aqueous, along with a specially developed propriety endcapping technology. Our unique patented approach provides a complete solution to ensure that Epic Polar XP is totally resistant to “phase collapse” under all mobile phase conditions and is able to deliver superior peak performance for amine containing compounds. In addition, Epic Polar XP can be used with any routinely used mobile phase modifier such as acetonitrile, methanol etc.
ES Industries has conducted extensive research to gain a fundamental insight into the “phase collapse” phenomenon. Our research has uncovered the key fundamental mechanism to explain and ultimately prevent “phase collapse”. The key facet to the mechanism is the relationship between mobile phase operating pressure and surface wettability of the bonded phase/silica particle.
Other column designs have been tried, with limited success, to prevent “phase collapse” from occurring. One of these approaches requires the use of a hydrophilic endcapping after the initial alkyl chain bonding. This approach falls short in two major areas. First, it requires a two-step bonding sequence, which subjects the final product to increased variability that can seriously jeopardize the reliability of HPLC methods. Secondly, the hydrophilic endcapping can beeasily damaged by the mobile phase which can considerably shorten column life and lead to unacceptable poor performance for routine chromatographic methods. Given these two major limitations we did not pursue the development of any hydrophilic endcapping approach in the development of a “phase collapse” resistant product.
Our patented silane allows the bonded alkyl chains to remain fully extended in the mobile phase even under highly aqueous conditions. To obtain high aqueous stability and maximum hydrophobic interaction Epic Polar XP relies on our specially developed silane which is bonded to ultra high purity synthetically produced spherical silica. This silane contains an ether linkage placed near the point of attachment to the silica base particle. This ether group is polar enough to allow water to penetrate and hydrate the silica surface preventing the self association of the hydrophobic alkyl chains. This layer of hydration permits the maximum interaction of the alkyl chains with the analytes of interest and prevents any phase collapse. The superior performance of Epic Polar XP not only includes our bonded ODS ether linked groups but a newly developed proprietary endcapping technique that delivers superior peak shape performance for amine containing compounds. This combination of our ether linked technology and proprietary endcapping technology enables the chromatographer to obtain high performance separation with any mobile phase condition.
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