ES Notes April 2009

As previewed last month - we will be featuring in this months issue a presentation given at Pittcon 2009 and the North Jersey Chromatography Discussion Group meetings titled "Strategies for the Chromatographic Separation and Analysis of Polar Compounds using HPLC".

In this issue we are also featuring a broad range of unique speciality SFC phases which are listed in the analytical columns format and are also available in semi-preparative and preparative sizes.

As always we hope you find ESNotes to be technically informative and practically useful.

For our international customers have you tested our dynamic translation tool yet? Visit the ES web site and click on the translate button - permitting you to perform "on the fly" translations of our Web Site, applications and past ESNotes to your native language with the click of a button...

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Strategies for the Chromatographic Separation and Analysis of Polar Compounds using HPLC

The complete characterization and analysis of many materials is critical for pharmaceutical, food and environmental samples.  These samples are composed of a wide variety of chemical moieties including both non-polar and polar compounds.  The analysis of the polar compounds has proven to be most challenging to accurately determine.   

The analysis of polar compounds via routine HPLC analysis has been deficient in several main aspects including poor retention, unacceptable low k' values and poor peak shapes.  However, the recent introduction of several new HPLC stationary phases has provided an improved format for the analysis of polar compounds. 

These new HPLC stationary phases include high aqueous stable phases, HILIC (hydrophilic interaction chromatography) phases and polar embedded phases. 

It is the focus of this presentation to compare and contrast the new HPLC stationary phases developed for the analysis of polar compounds.   Please visit our website to download the complete presentation.

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Speciality SFC Columns

The development of new SFC stationary phases has been a key area of development for ES Industries.  ES Industries is pleased to present a list of eleven of our SFC column chemistries that have shown unique selectivity. We are particularly excited by the separation performance of Chromegabond Pyridyl Amide, Chromegabond Amino Phenyl and Chromegabond NO2.  All of these materials are also available in semi-preparative and preparative sizes.

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SonomaTM  HPLC Columns

The SonomaTM line is an equivalent to Phenomenex LunaTM. There are several different phases available in the Sonoma line The most popular is the C18(2). ES Industries offers this exact type HPLC column in 3µ and 5µ sizes with bulk and preparative material available. Excellent efficiencies, peak shape and resolution are obtained for virtually all Luna HPLC applications. A range of particle sizes offers amazing versatility for capillary and LC/MS to prep and process scale applications. ES Industries has developed a line of Sonoma stationary phases equivalent to Phenomenex Luna stationary phases. The new Sonoma phases include C18, C8, HILIC, NH2, SCX and PFP. With these new additions our current Sonoma offerings are:

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P.S. Watch in the May 2009 issue of ESNotes for -

SunriseTM an alternative to Waters Sunfire HPLC phases

Epic Polar the ultimate C18 column for high aqueous HPLC separations

An overview of polar bonded phases for normal phase HPLC

David Kohler
President
ES Industries, Inc.